Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
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The SpectraMax Fluorescence Intensity Filter Kit delivers five exchangeable optical filters for fluorescence, luminescence, and time-resolved fluorescence (TRF) assays on compatible multi-mode microplate readers. The kit includes an emission 660 nm filter with a 40 nm bandwidth plus additional excitation and emission filters to support common assays.
- Includes five exchangeable filters covering common fluorescence wavelengths from ~400-700 nm.
- Contains an emission 660 nm filter with a 40 nm full bandwidth for near-IR fluorescence detection.
- Supports fluorescence intensity, luminescence, and time-resolved fluorescence assay modes.
- Allows quick filter exchange to adapt to different assay requirements.
- Designed for use with compatible multi-mode microplate readers to expand detection flexibility.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Replacement fluorescence emission filter with a 650 nm center wavelength and 40 nm bandwidth, designed for use in Molecular Devices FilterMax F-series multi-mode microplate readers to collect far-red fluorescence emission.
- Center wavelength 650 nm.
- Bandwidth 40 nm for selective far-red detection.
- Designed for installation in filter slide assemblies.
- Enables assays requiring far-red emission collection with reduced background.
- Replacement part for multi-mode microplate reader fluorescence modules.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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