Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Molecular Devices SpectraTest Validation Plate, FL1, Calibration Certified, Reader Compatible
The SpectraTest FL1 Fluorescence Validation Plate is a calibrated reference tool used to validate the optical performance of FL1-channel fluorescence-capable microplate readers. It verifies sensitivity, wavelength accuracy, RFU linearity, PMT matching, and other fluorescence performance parameters and ships with a certificate of calibration and protective storage.
- Validates fluorescence sensitivity, wavelength accuracy, PMT matching, and RFU linearity.
- Includes calibrated fluorescent and non-fluorescent reference strips and didymium glass.
- Supplied with a certificate of calibration and protective storage sleeve.
- Compatible with a wide range of microplate readers for FL1-channel testing.
- Recommended annual recertification to maintain traceable calibration.
Molecular Devices SpectraMax iD5 Microplate Reader, Bottom Luminescence, 6-384 Well
The SpectraMax iD5 is a multi-mode microplate reader configurable with a bottom luminescence read mode for sensitive luminescence assays. It supports absorbance, fluorescence, luminescence, and time-resolved measurements across 6-384 well plates, and accepts optional injectors and micro-volume or blot accessories to expand workflow capability.
- Five detection modes including absorbance, fluorescence, and luminescence.
- Compatible with 6-384 well plate formats.
- Optional injector system for on-plate reagent delivery.
- Integrated touchscreen with onboard data acquisition software.
- NFC-enabled filter identification for automated filter selection.
- Temperature control up to 66°C and multiple shaking modes.
Molecular Devices Luminescence Filter, 410 nm, BW 80 nm
This Molecular Devices luminescence emission filter (with holder) provides a 410 nm center wavelength with an 80 nm bandwidth for luminescence detection in microplate assays. It is listed as an accessory for SpectraMax iD series multi-mode microplate readers and is commonly used as a donor emission filter in assays such as BRET2.
- Provides a 410 nm center wavelength with an 80 nm bandwidth
- Includes holder for secure installation in filter slot
- Optimized for luminescence emission detection
- Used as donor emission filter in luminescence assays
- Compatible with multi-mode microplate reader filter systems
Sigma Aldrich Fine Chemicals Biosciences Duolink(R) In Situ Wash Buffers, Fluorescence | 20L
Duolink(R) In Situ Wash Buffers, Fluorescence | 20L
Revvity Health Sciences Inc Revvity HTRF Anti-6His mAb d2-Conjugate, 20,000 Assay Points, TR-FRET
This HTRF Anti-6His monoclonal antibody d2-Conjugate is a d2-labeled anti-6His antibody, specifically designed for capturing 6His-tagged proteins in protein/protein interaction assays.
- Designed for capturing 6His-tagged proteins.
- Used in protein/protein interaction assays.
- Features 20,000 assay points.
- Utilizes protein-protein interaction application.
- HTRF detection modality.
- Targets 6HIS.
- Binding assay target class.
- TR-FRET technology.
- Shipped ambient.
Alkali Scientific ASI™ Confocal Dish 35 x10mm, Clear, Easy-Grip, Insert Type, Sterile, PS/Glass, Non-Treated (Non-Charged/ Hydrophobic), 500/Cs
ASI™ product with a coverglass bottom is easy to use and facilitates preparation of cells for microscopic analysis. Optically clear with low background fluorescence coverglass was used. Ideal for use in high resolution and inverted microscopy, fluorescence imaging in live cells, confocal microscopy, phase contrast microscopy and micro-manipulations. ▪ Dish size : 35 mm Plate size: 6well plate(Cat.NO. 30106, 30206)▪ Hole size : 13 Øor 20 Ø▪ Coverglass size : 22 x 22 mm(adhesion type, hole 13 Ø), 24 x 24(insert and adhesion type, hole 20 Ø)▪ Coverglass thickness : 0.13 ~ 0.16 mm▪ External grip 35 mm dish are available▪ Black color 35mm dish is also available▪ Sterilized by gamma irradiation▪ Polystyrene
Alkali Scientific ASI™ 6-Well Confocal Plate (35m), Clear, Adhesion Type, Sterile, 4/Cs, PS/Glass, Non-Treated (Non-Charged/ Hydrophobic), 100/Cs, 22 x 22 x 0.13mm, Hole 3.14mm
ASI™ confocal products allow researchers to acquire high resolution microscopic images of cells in 35 mm culture dishes or 6 well plates. Low auto-fluorescence glass and FLux were used, ideal for uses of these products are confocal microscopy, phase contrast microscopy, live cell imaging and micro-manipulations. Surface materials for confocal products: Glass: naturally charged, good growth surface for adherent cellsFLux: surface modified plastic film (188 μm thick), optimal for confocal microscopy ▪ Bottom materials: Glass/FLux (no surface treatment for glass bottom)▪ Dish color: Clear▪ Dish size: 35 Ø▪ Plate size: 6well Plate▪ Hole size: 13 Ø / 20 Ø / 25Ø▪ Non – pyrogenic▪ Non – cytotoxic▪ DNase / RNase – free▪ Human DNA – free
Advanced Instruments Inc MVS VERIFICATION PLATES-N 25BX
MVS Verification Plate; 96-well; Optical quality; Flat bottom plates which are dimensionally characterized and bar coded with lot number and a unique identifier; 25/PK
Bio Rad Laboratories SINGLE-COMPONENT TMB PEROXIDAS
Kit, Substrate; Bio-Rad; TMB Peroxidase EIA; Accessories for iMark Microplate Absorbance Reader; 100mLsolution
Electron Microscopy Sciences NIGHTSEA™ Stereo Fluorescence Add-On Light and Filter Set, Ultra Violet
Additional combinations excitation/ emission wavelength combination can be added NIGHTSEA™ Stereo Microscope Fluorescence Viewing system by purchasing a kit that consists of a light head, barrier filter, and viewing shield. These three elements can be removed and replaced in seconds, and color coding ensures that you are using the right combination. Sets are named and color coded for the color of the excitation light, not the color of the emitted fluorescence.
Color: Ultra Violet
Excitation: 360-380nm
Emission filter: 415 nm longpass
Fluorophores: DAPI
Revvity Health Sciences Inc HTRF Human IRF5 Detection Kit, 500 Assay Points
HTRF Human IRF5 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-TYK2 (Tyr1054/1055) Detection Kit, 500 Assay Points
HTRF Human Phospho-TYK2 (Tyr1054/1055) Detection Kit, 500 Assay Points
Molecular Devices Fluorescence Filter, 625 nm, 35 nm BW
Replacement fluorescence emission filter supplied with a mounting holder for use in compatible Molecular Devices microplate readers. The filter provides a red-region emission band centered at 625 nm with a 35 nm full bandwidth, suitable for fluorescence intensity and time-resolved fluorescence assays.
- Center wavelength 625 nm.
- Bandwidth 35 nm full width.
- Includes mounting holder for slide insertion.
- Optimized for red-emission fluorescence detection.
- Intended for use in microplate readers that accept plug-in filters.